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1 change: 1 addition & 0 deletions CHANGELOG.md
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Expand Up @@ -15,6 +15,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
- [PR #1490](https://github.com/nf-core/rnaseq/pull/1490) - Make genomic FASTA input optional
- [PR #1496](https://github.com/nf-core/rnaseq/pull/1496) - Template update for nf-core/tools v3.2.0
- [PR #1499](https://github.com/nf-core/rnaseq/pull/1499) - Bump MultiQC module to 1.27
- [PR #1508](https://github.com/nf-core/rnaseq/pull/1508) - Fix missing Bracken results in the MultiQC report

# 3.18.0 - 2024-12-19

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2 changes: 1 addition & 1 deletion docs/output.md
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Expand Up @@ -697,7 +697,7 @@ The plot on the left hand side shows the standard PC plot - notice the variable

</details>

[Kraken2](https://ccb.jhu.edu/software/kraken2/) is a taxonomic classification tool that uses k-mer matches paired with a lowest common ancestory (LCA) algorithm to classify species reads. [Bracken](https://ccb.jhu.edu/software/bracken/) is a statistical method to generate abundance estimates based off of the Kraken2 output. These algorithms are run on unaligned sequences to detect potential contamination of samples. MultiQC reports the top 5 taxon members detected at the level of classification used for Bracken, with toggles available for higher taxonomic levels. If Bracken is skipped, MultiQC will report the top 5 species detected by Kraken2.
[Kraken2](https://ccb.jhu.edu/software/kraken2/) is a taxonomic classification tool that uses k-mer matches paired with a lowest common ancestory (LCA) algorithm to classify species reads. [Bracken](https://ccb.jhu.edu/software/bracken/) is a statistical method to generate abundance estimates based off of the Kraken2 output. These algorithms are run on unaligned sequences to detect potential contamination of samples. MultiQC reports the top 5 taxon members detected at the level of classification used for Bracken, with toggles available for higher taxonomic levels. Because MultiQC no longer has a separate Bracken module, results for Bracken will appear under the Kraken heading in the MultiQC report. If Bracken is skipped, MultiQC will report the top 5 species detected by Kraken2.

![MultiQC - Bracken top species plot](images/bracken-top-n-plot.png)

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9 changes: 2 additions & 7 deletions workflows/rnaseq/assets/multiqc/multiqc_config.yml
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Expand Up @@ -97,7 +97,6 @@ run_modules:
- rseqc
- qualimap
- kraken
- bracken

# Order of modules

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fn: "*.flagstat"
samtools/idxstats:
fn: "*.idxstats*"

kraken:
fn: "*report.txt"

bracken:
fn: "*.kraken2.report_bracken.txt"

- fn: "*.kraken2.report.txt"
- fn: "*.kraken2.report_bracken.txt"
rseqc/bam_stat:
fn: "*.bam_stat.txt"
rseqc/gene_body_coverage:
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