FastQC report duplication levels consultation

[miryamcarrillo98]

Hi,

I have some trouble interpreting the FastQC Report. What might be the most plausible reasons to the high duplicated sequences? Why don't I get the overrepresented sequences in FastQC Report even though I have more than 80% duplicated sequences?

Thank you.

[s-andrews]

Interpreting these plots isn't always easy, and the expected profile is quite different depending on the type of library you have. There's a nice write up on QCFail which goes through the different types of duplication and how to diagnose them.