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2 changes: 2 additions & 0 deletions CHANGELOG.md
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Expand Up @@ -9,6 +9,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0

Special thanks to the following for their contributions to the release:

- [Ahwan Pandey](https://github.com/ahwanpandey)
- [Elad Herzog](https://github.com/EladH1)
- [Emily Miyoshi](https://github.com/emilymiyoshi)
- [Pontus Höjer](https://github.com/pontushojer)
Expand All @@ -29,6 +30,7 @@ Special thanks to the following for their contributions to the release:
- [PR #1628](https://github.com/nf-core/rnaseq/pull/1628) - Template update for nf-core/tools v3.5.1
- [PR #1630](https://github.com/nf-core/rnaseq/pull/1630) - Fix arm64 profile to use pre-built ARM containers and update documentation
- [PR #1631](https://github.com/nf-core/rnaseq/pull/1631) - Fix bbsplit index staging by using symlinks instead of full copy
- [PR #1635](https://github.com/nf-core/rnaseq/pull/1635) - Fix `--gtf_extra_attributes` to support multiple comma-separated values and correct deprecated parameter name in docs ([#1626](https://github.com/nf-core/rnaseq/issues/1626))

## [[3.21.0](https://github.com/nf-core/rnaseq/releases/tag/3.21.0)] - 2025-09-18

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2 changes: 1 addition & 1 deletion docs/usage.md
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Expand Up @@ -358,7 +358,7 @@ Remember to note the genome and annotation versions as well as the versions of t

If you are using [GENCODE](https://www.gencodegenes.org/) reference genome files please specify the `--gencode` parameter because the format of these files is slightly different to ENSEMBL genome files:

- The `--gtf_group_features_type` parameter will automatically be set to `gene_type` as opposed to `gene_biotype`, respectively.
- The `--featurecounts_group_type` parameter will automatically be set to `gene_type` as opposed to `gene_biotype`, respectively.
- If you are running Salmon, the `--gencode` flag will also be passed to the index building step to overcome parsing issues resulting from the transcript IDs in GENCODE fasta files being separated by vertical pipes (`|`) instead of spaces (see [this issue](https://github.com/COMBINE-lab/salmon/issues/15)).

As well as the standard annotations, GENCODE also provides "basic" annotations, which include only representative transcripts, but we do not recommend using these.
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2 changes: 1 addition & 1 deletion modules.json
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Expand Up @@ -37,7 +37,7 @@
},
"custom/tx2gene": {
"branch": "master",
"git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
"git_sha": "e0bdf8637721e27792a38c6b622f0a2345f3cbc9",
"installed_by": ["modules", "quantify_pseudo_alignment"]
},
"dupradar": {
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39 changes: 22 additions & 17 deletions modules/nf-core/custom/tx2gene/meta.yml

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17 changes: 12 additions & 5 deletions modules/nf-core/custom/tx2gene/templates/tx2gene.py

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46 changes: 45 additions & 1 deletion modules/nf-core/custom/tx2gene/tests/main.nf.test

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33 changes: 33 additions & 0 deletions modules/nf-core/custom/tx2gene/tests/main.nf.test.snap

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