v0.9.7

Picasso 0.9.7 is here

Changelog

Important updates:

• Windows one-click-installer allows for selecting only a subset of Picasso modules to install
• Added ToRaw and Nanotron to one-click-installer
• Experimental One-click-installer for macOS (only for Apple Silicon), see here

Small improvements:

• Adjusted the config.yaml and plugins instructions for the one-click-installer Picasso release (new Pyinstaller stores everything in the _internal folder)
• G5M output can save more columns (if present in the input localizations)
• Further enhancement of G5M documentation
• Render GUI: implemented filter by number of localizations for multichannel data
• Render GUI: allow removal of any column from localizations, not only group
• Filter GUI: allow removal of any column from localizations
• REQUIRED_COLUMNS moved from picasso.localize to picasso.lib

Bug fixes:

• Fixed basic frame analysis in SMLM clusterer
• Fixed labels of the vertical lines in the subcluster test plot
• Fixed automatic Localize loading/unloading z-calibration paths when changing cameras
• Fixed rel_sigma_z in G5M (previously incorrectly divided by pixel size)
• Fixed G5M molmap lpz output
• Fixed loading square picks in Render
• Fixed appearance of the Apply expression dialog in Render for files with many columns
• Fixed initial x, y and N in LQ Gaussian fitting (might results in faster convergence and slightly different (<< NeNA) results) (#616)
• Fixed picking circular regions around left and top edges of the FOV

Notes

‼️ If installed in Program Files, Render and Localize may not be available for non-administrator users. Therefore, we recommend installing Picasso outside of Program Files. The current default location is C:\Picasso.
⚠️ When using Windows installer, camera config file needs to be moved to C:\Picasso\_internal\picasso. Previously under C:\Picasso\picasso.
⚠️ Windows Safety features and Windows Defender may ask multiple times for permission during the installation and download.