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Hi,
I've just installed cruzdb in Python 2.7. I found that the first exon was missed from this gene A4GNT, NM_016161. Here is how I fetched the sequence:
g = Genome(db="hg19")
gene='A4GNT'
gene_obj = g.refGene.filter_by(name2=gene).first()
print(len(gene_obj.mrna_sequence))
got 2, but RefSeq shows 3 exons
print(len(gene_obj.mrna_sequence[0]))
1161
print(len(gene_obj.mrna_sequence[1]))
434
print(len(gene_obj.mrna_sequence[2]))
index out of range error
I cross checked NM_016161 in NCBI, it seems the 1st exon (176 bps long) was not included.
Any help appreciated.
Eric.
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