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2 changes: 1 addition & 1 deletion README.md
Original file line number Diff line number Diff line change
Expand Up @@ -91,7 +91,7 @@ will run assembly with only Illumina paired end reads from files /path_to/pe_R1.

This command will run a hybrid assembly, correcting Nanopore reads with Illumina data first. Ilumina paired end reads files must be fastq, can be gzipped, and Nanopore/PacBio data files for the -r option can be fasta or fastq and can be gzipped.

For bigger projects that use Ullimina data from multiple instrument runs, Illumina mate pairs, legacy Sanger data and/or need parameter adjustments, there is a more advanced mode. To use it, create a configuration file which contains the location of the compiled assembler, the location of the data and some parameters. Copy in your assembly directory the template configuration file `/install_path/sr_config_example.txt` which was created by the installer with the correct paths to the freshly compiled software and with reasonable parameters. Most assembly projects should only need to set the paths to the input data in the example configuration. More detailed description of the configuration is in the next section.
For bigger projects that use Illumina data from multiple instrument runs, Illumina mate pairs, legacy Sanger data and/or need parameter adjustments, there is a more advanced mode. To use it, create a configuration file which contains the location of the compiled assembler, the location of the data and some parameters. Copy in your assembly directory the template configuration file `/install_path/sr_config_example.txt` which was created by the installer with the correct paths to the freshly compiled software and with reasonable parameters. Most assembly projects should only need to set the paths to the input data in the example configuration. More detailed description of the configuration is in the next section.

Second, run the `masurca` script which will generate from the configuration file a shell script `assemble.sh`. This last script is the main driver of the assembly.

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