diff --git a/CHANGELOG.md b/CHANGELOG.md
index 377ef036230a..f97dc1f72470 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -25,6 +25,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 ### Fixed
 
 - Fix class extraction followed by `(` in Pug ([#17320](https://github.com/tailwindlabs/tailwindcss/pull/17320))
+- Ensure `@keyframes` for theme animations are emitted if they are referenced following a comma
 - Vite: Ensure that updates to an imported CSS file are properly propagated after updating templates ([#17347](https://github.com/tailwindlabs/tailwindcss/pull/17347))
 - Pre process `Slim` templates embedded in Ruby files ([#17336](https://github.com/tailwindlabs/tailwindcss/pull/17336))
 - Error when input and output files resolve to the same file when using the CLI ([#17311](https://github.com/tailwindlabs/tailwindcss/pull/17311))
diff --git a/packages/tailwindcss/src/ast.ts b/packages/tailwindcss/src/ast.ts
index 83674d1148e9..e13e19743209 100644
--- a/packages/tailwindcss/src/ast.ts
+++ b/packages/tailwindcss/src/ast.ts
@@ -310,8 +310,8 @@ export function optimizeAst(ast: AstNode[], designSystem: DesignSystem) {
 
       // Track used animation names
       if (node.property === 'animation') {
-        let parts = node.value.split(/\s+/)
-        for (let part of parts) usedKeyframeNames.add(part)
+        for (let keyframeName of extractKeyframeNames(node.value))
+          usedKeyframeNames.add(keyframeName)
       }
 
       parent.push(node)
@@ -438,8 +438,8 @@ export function optimizeAst(ast: AstNode[], designSystem: DesignSystem) {
       )
       if (variableUsed) {
         if (declaration.property.startsWith(designSystem.theme.prefixKey('--animate-'))) {
-          let parts = declaration.value!.split(/\s+/)
-          for (let part of parts) usedKeyframeNames.add(part)
+          for (let keyframeName of extractKeyframeNames(declaration.value!))
+            usedKeyframeNames.add(keyframeName)
         }
 
         continue
@@ -605,3 +605,7 @@ function isVariableUsed(
 
   return false
 }
+
+function extractKeyframeNames(value: string): string[] {
+  return value.split(/[\s,]+/)
+}
diff --git a/packages/tailwindcss/src/index.test.ts b/packages/tailwindcss/src/index.test.ts
index ce3dd9d47182..25d30f2ea670 100644
--- a/packages/tailwindcss/src/index.test.ts
+++ b/packages/tailwindcss/src/index.test.ts
@@ -1939,6 +1939,49 @@ describe('Parsing theme values from CSS', () => {
     `)
   })
 
+  // https://github.com/tailwindlabs/tailwindcss/issues/17332
+  test('extracts keyframe names followed by comma', async () => {
+    expect(
+      await compileCss(
+        css`
+          @theme {
+            --animate-test: 500ms both fade-in, 1000ms linear 500ms spin infinite;
+
+            @keyframes fade-in {
+              from {
+                opacity: 0%;
+              }
+              to {
+                opacity: 100%;
+              }
+            }
+          }
+
+          @tailwind utilities;
+        `,
+        ['animate-test'],
+      ),
+    ).toMatchInlineSnapshot(`
+      ":root, :host {
+        --animate-test: .5s both fade-in, 1s linear .5s spin infinite;
+      }
+
+      .animate-test {
+        animation: var(--animate-test);
+      }
+
+      @keyframes fade-in {
+        from {
+          opacity: 0;
+        }
+
+        to {
+          opacity: 1;
+        }
+      }"
+    `)
+  })
+
   test('keyframes outside of `@theme are always preserved', async () => {
     expect(
       await compileCss(