diff --git a/Cargo.lock b/Cargo.lock
index 3956b012f64d4..ae446c1f38fdd 100644
--- a/Cargo.lock
+++ b/Cargo.lock
@@ -13320,6 +13320,7 @@ dependencies = [
  "sp-io",
  "sp-keystore",
  "sp-runtime",
+ "sp-state-machine",
  "sp-tracing 16.0.0",
  "sp-version",
  "substrate-bn",
diff --git a/prdoc/pr_10297.prdoc b/prdoc/pr_10297.prdoc
new file mode 100644
index 0000000000000..18f5206493f5d
--- /dev/null
+++ b/prdoc/pr_10297.prdoc
@@ -0,0 +1,10 @@
+title: "pallet_revive: use real storage for block number when building the eth genesis block"
+doc:
+- audience: Runtime Dev
+  description: |-
+    When building the eth genesis block, query the real storage item that stores the block number instead of using zero.
+    If the chainspec does not customise it, it will remain zeroed. Was previously fixed in https://github.com/paritytech/polkadot-sdk/pull/10225
+    and regression introduced in https://github.com/paritytech/polkadot-sdk/pull/10271. Adds a unit test and comment to prevent further regressions.
+crates: 
+- name: pallet-revive
+  bump: patch
diff --git a/substrate/frame/revive/Cargo.toml b/substrate/frame/revive/Cargo.toml
index 3c655612e490c..d51d6db753546 100644
--- a/substrate/frame/revive/Cargo.toml
+++ b/substrate/frame/revive/Cargo.toml
@@ -82,6 +82,7 @@ pallet-timestamp = { workspace = true, default-features = true }
 pallet-utility = { workspace = true, default-features = true }
 proptest = { workspace = true }
 sp-keystore = { workspace = true, default-features = true }
+sp-state-machine = { workspace = true }
 sp-tracing = { workspace = true, default-features = true }
 
 [features]
@@ -127,6 +128,7 @@ std = [
 	"sp-io/std",
 	"sp-keystore/std",
 	"sp-runtime/std",
+	"sp-state-machine/std",
 	"sp-version/std",
 	"subxt-signer",
 ]
diff --git a/substrate/frame/revive/src/lib.rs b/substrate/frame/revive/src/lib.rs
index e8c9c638bc59c..3b6f884711f7a 100644
--- a/substrate/frame/revive/src/lib.rs
+++ b/substrate/frame/revive/src/lib.rs
@@ -829,7 +829,11 @@ pub mod pallet {
 			}
 
 			// Build genesis block
-			block_storage::on_finalize_build_eth_block::<T>(0u32.into());
+			block_storage::on_finalize_build_eth_block::<T>(
+				// Make sure to use the block number from storage instead of the hardcoded 0.
+				// This enables testing tools like anvil to customise the genesis block number.
+				frame_system::Pallet::<T>::block_number(),
+			);
 
 			// Set debug settings.
 			if let Some(settings) = self.debug_settings.as_ref() {
diff --git a/substrate/frame/revive/src/tests.rs b/substrate/frame/revive/src/tests.rs
index f09afd070789a..6b992919600f0 100644
--- a/substrate/frame/revive/src/tests.rs
+++ b/substrate/frame/revive/src/tests.rs
@@ -50,7 +50,7 @@ use sp_keystore::{testing::MemoryKeystore, KeystoreExt};
 use sp_runtime::{
 	generic::Header,
 	traits::{BlakeTwo256, Convert, IdentityLookup, One},
-	AccountId32, BuildStorage, MultiAddress, MultiSignature, Perbill,
+	AccountId32, BuildStorage, MultiAddress, MultiSignature, Perbill, Storage,
 };
 
 pub type Address = MultiAddress<AccountId32, u32>;
@@ -439,6 +439,7 @@ pub struct ExtBuilder {
 	storage_version: Option<StorageVersion>,
 	code_hashes: Vec<sp_core::H256>,
 	genesis_config: Option<crate::GenesisConfig<Test>>,
+	genesis_state_overrides: Option<Storage>,
 }
 
 impl Default for ExtBuilder {
@@ -448,6 +449,7 @@ impl Default for ExtBuilder {
 			storage_version: None,
 			code_hashes: vec![],
 			genesis_config: Some(crate::GenesisConfig::<Test>::default()),
+			genesis_state_overrides: None,
 		}
 	}
 }
@@ -469,10 +471,19 @@ impl ExtBuilder {
 	pub fn set_associated_consts(&self) {
 		EXISTENTIAL_DEPOSIT.with(|v| *v.borrow_mut() = self.existential_deposit);
 	}
+	pub fn with_genesis_state_overrides(mut self, storage: Storage) -> Self {
+		self.genesis_state_overrides = Some(storage);
+		self
+	}
 	pub fn build(self) -> sp_io::TestExternalities {
 		sp_tracing::try_init_simple();
 		self.set_associated_consts();
-		let mut t = frame_system::GenesisConfig::<Test>::default().build_storage().unwrap();
+		let mut t = self.genesis_state_overrides.unwrap_or_default();
+
+		frame_system::GenesisConfig::<Test>::default()
+			.assimilate_storage(&mut t)
+			.unwrap();
+
 		let checking_account = Pallet::<Test>::checking_account();
 
 		pallet_balances::GenesisConfig::<Test> {
diff --git a/substrate/frame/revive/src/tests/block_hash.rs b/substrate/frame/revive/src/tests/block_hash.rs
index 68685481c6b5e..d83b55916e9be 100644
--- a/substrate/frame/revive/src/tests/block_hash.rs
+++ b/substrate/frame/revive/src/tests/block_hash.rs
@@ -20,7 +20,7 @@
 use crate::{
 	evm::{block_hash::EthereumBlockBuilder, fees::InfoT, Block, TransactionSigned},
 	test_utils::{builder::Contract, deposit_limit, ALICE},
-	tests::{assert_ok, builder, Contracts, ExtBuilder, RuntimeOrigin, Test},
+	tests::{assert_ok, builder, Contracts, ExtBuilder, RuntimeOrigin, System, Test, Timestamp},
 	BalanceWithDust, Code, Config, EthBlock, EthBlockBuilderFirstValues, EthBlockBuilderIR,
 	EthereumBlock, Pallet, ReceiptGasInfo, ReceiptInfoData,
 };
@@ -31,6 +31,7 @@ use frame_support::traits::{
 	Hooks,
 };
 use pallet_revive_fixtures::compile_module;
+use sp_state_machine::BasicExternalities;
 
 #[test]
 fn on_initialize_clears_storage() {
@@ -52,6 +53,26 @@ fn on_initialize_clears_storage() {
 	});
 }
 
+#[test]
+fn genesis_block_number_and_timestamp_fetched_from_storage() {
+	let mut ext = BasicExternalities::new_empty();
+	ext.execute_with(|| {
+		System::set_block_number(10);
+		Timestamp::set_timestamp(10000000);
+	});
+	let storage = ext.into_storages();
+
+	ExtBuilder::default()
+		.with_genesis_state_overrides(storage)
+		.build()
+		.execute_with(|| {
+			let block = EthereumBlock::<Test>::get();
+			// The timestamp is divided by 1000 (converted to seconds)
+			assert_eq!(block.timestamp, 10000.into());
+			assert_eq!(block.number, 10.into());
+		});
+}
+
 #[test]
 fn transactions_are_captured() {
 	let (binary, _) = compile_module("dummy").unwrap();