diff --git a/CHANGELOG.md b/CHANGELOG.md
index 7c5308fcf7a..8c3430e1a75 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -15,6 +15,7 @@ This project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.htm
 ### Fixed
 
 - Fix panic when passing nil `TracerProvider` or `MeterProvider` to `WithTracerProvider` or `WithMeterProvider` in `go.opentelemetry.io/contrib/instrumentation/google.golang.org/grpc/otelgrpc`. (#8323)
+- `Transport` in `go.opentelemetry.io/contrib/instrumentation/net/http/otelhttp` now supports reading request body multiple times for subsequent requests that reuse `http.Request`. (#8352)
 
 ### Removed
 
diff --git a/instrumentation/net/http/otelhttp/transport.go b/instrumentation/net/http/otelhttp/transport.go
index 514ae6753b7..1d2f4022116 100644
--- a/instrumentation/net/http/otelhttp/transport.go
+++ b/instrumentation/net/http/otelhttp/transport.go
@@ -5,6 +5,7 @@ package otelhttp // import "go.opentelemetry.io/contrib/instrumentation/net/http
 
 import (
 	"context"
+	"fmt"
 	"io"
 	"net/http"
 	"net/http/httptrace"
@@ -83,6 +84,8 @@ func defaultTransportFormatter(_ string, r *http.Request) string {
 // RoundTrip creates a Span and propagates its context via the provided request's headers
 // before handing the request to the configured base RoundTripper. The created span will
 // end when the response body is closed or when a read from the body returns io.EOF.
+// If GetBody returns an error, the error is reported via otel.Handle and the request
+// continues with the original Body.
 func (t *Transport) RoundTrip(r *http.Request) (*http.Response, error) {
 	requestStartTime := time.Now()
 	for _, f := range t.filters {
@@ -117,11 +120,22 @@ func (t *Transport) RoundTrip(r *http.Request) (*http.Response, error) {
 
 	r = r.Clone(ctx) // According to RoundTripper spec, we shouldn't modify the origin request.
 
-	// if request body is nil or NoBody, we don't want to mutate the body as it
+	// GetBody is preferred over direct access to Body if the function is set.
+	// If the resulting body is nil or is NoBody, we don't want to mutate the body as it
 	// will affect the identity of it in an unforeseeable way because we assert
 	// ReadCloser fulfills a certain interface and it is indeed nil or NoBody.
-	bw := request.NewBodyWrapper(r.Body, func(int64) {})
-	if r.Body != nil && r.Body != http.NoBody {
+	body := r.Body
+	if r.GetBody != nil {
+		b, err := r.GetBody()
+		if err != nil {
+			otel.Handle(fmt.Errorf("http.Request GetBody returned an error: %w", err))
+		} else {
+			body = b
+		}
+	}
+
+	bw := request.NewBodyWrapper(body, func(int64) {})
+	if body != nil && body != http.NoBody {
 		r.Body = bw
 	}
 
diff --git a/instrumentation/net/http/otelhttp/transport_test.go b/instrumentation/net/http/otelhttp/transport_test.go
index d4d20b2bfa5..bb03883c682 100644
--- a/instrumentation/net/http/otelhttp/transport_test.go
+++ b/instrumentation/net/http/otelhttp/transport_test.go
@@ -20,6 +20,7 @@ import (
 
 	"github.com/stretchr/testify/assert"
 	"github.com/stretchr/testify/require"
+	"go.opentelemetry.io/otel"
 	"go.opentelemetry.io/otel/attribute"
 	"go.opentelemetry.io/otel/codes"
 	"go.opentelemetry.io/otel/propagation"
@@ -1114,3 +1115,90 @@ func BenchmarkTransportRoundTrip(b *testing.B) {
 		})
 	}
 }
+
+func TestRequestBodyReuse(t *testing.T) {
+	var bodies []string
+	server := httptest.NewServer(http.HandlerFunc(func(w http.ResponseWriter, r *http.Request) {
+		body, err := io.ReadAll(r.Body)
+		assert.NoError(t, err)
+
+		defer r.Body.Close()
+		bodies = append(bodies, string(body))
+
+		w.WriteHeader(http.StatusOK)
+	}))
+	defer server.Close()
+
+	transport := NewTransport(http.DefaultTransport)
+	client := http.Client{Transport: transport}
+
+	body := bytes.NewBuffer([]byte("hello"))
+
+	req, err := http.NewRequestWithContext(t.Context(), http.MethodPost, server.URL, body)
+	require.NoError(t, err)
+
+	for range 2 {
+		resp, err := client.Do(req)
+		assert.NoError(t, err, "failed to perform http req")
+
+		_, err = io.Copy(io.Discard, resp.Body)
+		require.NoError(t, err)
+
+		err = resp.Body.Close()
+		require.NoError(t, err)
+	}
+
+	require.Len(t, bodies, 2, "expected exactly 2 req bodies")
+	assert.Equal(t, bodies[0], bodies[1], "req bodies do not match")
+}
+
+type testErrHandler struct {
+	err error
+}
+
+func (h *testErrHandler) Handle(err error) { h.err = err }
+
+func TestHandleErrorOnGetBodyError(t *testing.T) {
+	errHandler := otel.GetErrorHandler()
+
+	testErrHandler := &testErrHandler{}
+
+	otel.SetErrorHandler(testErrHandler)
+	defer func() {
+		otel.SetErrorHandler(errHandler)
+	}()
+
+	var receivedBody string
+
+	server := httptest.NewServer(http.HandlerFunc(func(w http.ResponseWriter, r *http.Request) {
+		b, err := io.ReadAll(r.Body)
+		assert.NoError(t, err)
+
+		receivedBody = string(b)
+
+		w.WriteHeader(http.StatusOK)
+	}))
+	defer server.Close()
+
+	body := "plain text body"
+
+	r, err := http.NewRequestWithContext(t.Context(), http.MethodPost, server.URL, strings.NewReader(body))
+	require.NoError(t, err)
+
+	getBodyErr := errors.New("GetBody error")
+	r.GetBody = func() (io.ReadCloser, error) {
+		return nil, getBodyErr
+	}
+
+	transport := NewTransport(http.DefaultTransport)
+	client := http.Client{Transport: transport}
+
+	resp, err := client.Do(r)
+	require.NotNil(t, resp)
+	require.NoError(t, err)
+	require.NoError(t, resp.Body.Close())
+
+	assert.Equal(t, http.StatusOK, resp.StatusCode)
+	assert.Equal(t, body, receivedBody, "should fallback to http.Request body on http.Request GetBody error")
+	assert.ErrorContains(t, testErrHandler.err, "http.Request GetBody returned an error: GetBody error")
+}