diff --git a/change/@fluentui-react-tree-3b5f955f-0166-4e3b-85dc-82dddef56b0c.json b/change/@fluentui-react-tree-3b5f955f-0166-4e3b-85dc-82dddef56b0c.json
new file mode 100644
index 0000000000000..556aa23d8b80c
--- /dev/null
+++ b/change/@fluentui-react-tree-3b5f955f-0166-4e3b-85dc-82dddef56b0c.json
@@ -0,0 +1,7 @@
+{
+  "type": "prerelease",
+  "comment": "bugfix: makes selector slot required when selection mode is defined",
+  "packageName": "@fluentui/react-tree",
+  "email": "bernardo.sunderhus@gmail.com",
+  "dependentChangeType": "patch"
+}
diff --git a/packages/react-components/react-tree/src/components/TreeItemLayout/useTreeItemLayout.tsx b/packages/react-components/react-tree/src/components/TreeItemLayout/useTreeItemLayout.tsx
index 8e195e14ca2fe..5687ddb7bf22e 100644
--- a/packages/react-components/react-tree/src/components/TreeItemLayout/useTreeItemLayout.tsx
+++ b/packages/react-components/react-tree/src/components/TreeItemLayout/useTreeItemLayout.tsx
@@ -80,6 +80,7 @@ export const useTreeItemLayout_unstable = (
     actions,
     expandIcon,
     selector: resolveShorthand(props.selector, {
+      required: selectionMode !== 'none',
       defaultProps: {
         checked,
         tabIndex: -1,