diff --git a/conformance/tests/basic/httproute_multiple_gateways_different_pools.go b/conformance/tests/basic/httproute_multiple_gateways_different_pools.go
new file mode 100644
index 000000000..251f55c81
--- /dev/null
+++ b/conformance/tests/basic/httproute_multiple_gateways_different_pools.go
@@ -0,0 +1,118 @@
+/*
+Copyright 2025 The Kubernetes Authors.
+
+Licensed under the Apache License, Version 2.0 (the "License");
+you may not use this file except in compliance with the License.
+You may obtain a copy of the License at
+
+    http://www.apache.org/licenses/LICENSE-2.0
+
+Unless required by applicable law or agreed to in writing, software
+distributed under the License is distributed on an "AS IS" BASIS,
+WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.
+See the License for the specific language governing permissions and
+limitations under the License.
+*/
+
+package basic
+
+import (
+	"net/http"
+	"testing"
+
+	"k8s.io/apimachinery/pkg/types"
+	"sigs.k8s.io/gateway-api/conformance/utils/suite"
+
+	"sigs.k8s.io/gateway-api-inference-extension/conformance/tests"
+	k8sutils "sigs.k8s.io/gateway-api-inference-extension/conformance/utils/kubernetes"
+	"sigs.k8s.io/gateway-api-inference-extension/conformance/utils/traffic"
+)
+
+func init() {
+	tests.ConformanceTests = append(tests.ConformanceTests, HTTPRouteMultipleGatewaysDifferentPools)
+}
+
+var HTTPRouteMultipleGatewaysDifferentPools = suite.ConformanceTest{
+	ShortName:   "HTTPRouteMultipleGatewaysDifferentPools",
+	Description: "Validates two HTTPRoutes on different Gateways successfully referencing different InferencePools and routes traffic accordingly.",
+	Manifests:   []string{"tests/basic/httproute_multiple_gateways_different_pools.yaml"},
+	Test: func(t *testing.T, s *suite.ConformanceTestSuite) {
+		const (
+			appBackendNamespace = "gateway-conformance-app-backend"
+			infraNamespace      = "gateway-conformance-infra"
+
+			primaryGatewayName    = "conformance-primary-gateway"
+			routeForPrimaryGWName = "route-for-primary-gateway"
+			primaryPoolName       = "primary-inference-pool"
+			primaryBackendPodName = "primary-inference-model-server"
+			primaryRoutePath      = "/test-primary-gateway"
+			primaryRouteHostname  = "primary.example.com"
+
+			secondaryGatewayName    = "conformance-secondary-gateway"
+			routeForSecondaryGWName = "route-for-secondary-gateway"
+			secondaryPoolName       = "secondary-inference-pool"
+			secondaryBackendPodName = "secondary-inference-model-server"
+			secondaryRoutePath      = "/test-secondary-gateway"
+			secondaryRouteHostname  = "secondary.example.com"
+		)
+
+		routeForPrimaryGWNN := types.NamespacedName{Name: routeForPrimaryGWName, Namespace: appBackendNamespace}
+		routeForSecondaryGWNN := types.NamespacedName{Name: routeForSecondaryGWName, Namespace: appBackendNamespace}
+		primaryPoolNN := types.NamespacedName{Name: primaryPoolName, Namespace: appBackendNamespace}
+		secondaryPoolNN := types.NamespacedName{Name: secondaryPoolName, Namespace: appBackendNamespace}
+		primaryGatewayNN := types.NamespacedName{Name: primaryGatewayName, Namespace: infraNamespace}
+		secondaryGatewayNN := types.NamespacedName{Name: secondaryGatewayName, Namespace: infraNamespace}
+
+		t.Run("Primary HTTPRoute, InferencePool, and Gateway path: verify status and traffic", func(t *testing.T) {
+			k8sutils.HTTPRouteAndInferencePoolMustBeAcceptedAndRouteAccepted(
+				t,
+				s.Client,
+				routeForPrimaryGWNN,
+				primaryGatewayNN,
+				primaryPoolNN,
+			)
+
+			primaryGwAddr := k8sutils.GetGatewayEndpoint(t, s.Client, s.TimeoutConfig, primaryGatewayNN)
+
+			traffic.MakeRequestAndExpectEventuallyConsistentResponse(
+				t,
+				s.RoundTripper,
+				s.TimeoutConfig,
+				primaryGwAddr,
+				traffic.Request{
+					Host:               primaryRouteHostname,
+					Path:               primaryRoutePath,
+					ExpectedStatusCode: http.StatusOK,
+					Backend:            primaryBackendPodName,
+					Namespace:          appBackendNamespace,
+				},
+			)
+		})
+
+		t.Run("Secondary HTTPRoute, InferencePool, and Gateway path: verify status and traffic", func(t *testing.T) {
+			k8sutils.HTTPRouteAndInferencePoolMustBeAcceptedAndRouteAccepted(
+				t,
+				s.Client,
+				routeForSecondaryGWNN,
+				secondaryGatewayNN,
+				secondaryPoolNN,
+			)
+
+			secondaryGwAddr := k8sutils.GetGatewayEndpoint(t, s.Client, s.TimeoutConfig, secondaryGatewayNN)
+
+			traffic.MakeRequestAndExpectEventuallyConsistentResponse(
+				t,
+				s.RoundTripper,
+				s.TimeoutConfig,
+				secondaryGwAddr,
+				traffic.Request{
+					Host:               secondaryRouteHostname,
+					Path:               secondaryRoutePath,
+					ExpectedStatusCode: http.StatusOK,
+					Backend:            secondaryBackendPodName,
+					Namespace:          appBackendNamespace,
+				},
+			)
+		})
+	},
+}
diff --git a/conformance/tests/basic/httproute_multiple_gateways_different_pools.yaml b/conformance/tests/basic/httproute_multiple_gateways_different_pools.yaml
new file mode 100644
index 000000000..b8ec2d114
--- /dev/null
+++ b/conformance/tests/basic/httproute_multiple_gateways_different_pools.yaml
@@ -0,0 +1,44 @@
+---
+apiVersion: gateway.networking.k8s.io/v1
+kind: HTTPRoute
+metadata:
+  name: route-for-primary-gateway
+  namespace: gateway-conformance-app-backend
+spec:
+  parentRefs:
+  - kind: Gateway
+    name: conformance-primary-gateway
+    namespace: gateway-conformance-infra
+  hostnames:
+  - "primary.example.com"
+  rules:
+  - backendRefs:
+    - group: inference.networking.x-k8s.io
+      kind: InferencePool
+      name: primary-inference-pool
+    matches:
+    - path:
+        type: PathPrefix
+        value: /test-primary-gateway
+---
+apiVersion: gateway.networking.k8s.io/v1
+kind: HTTPRoute
+metadata:
+  name: route-for-secondary-gateway
+  namespace: gateway-conformance-app-backend
+spec:
+  parentRefs:
+  - kind: Gateway
+    name: conformance-secondary-gateway
+    namespace: gateway-conformance-infra
+  hostnames:
+  - "secondary.example.com"
+  rules:
+  - backendRefs:
+    - group: inference.networking.x-k8s.io
+      kind: InferencePool
+      name: secondary-inference-pool
+    matches:
+    - path:
+        type: PathPrefix
+        value: /test-secondary-gateway