How to report the logfile to Windows when using DearPyGui?

[joybio]

I encountered a problem when trying to package a Python script written with DearPyGui. The script includes an executable file (muscle5.1.win64.exe) that I use within the Python code. When I run the script in PyCharm, it works fine. However, when I package it into an executable file, it crashes (flashes back)

Thank you!

[joybio]

Here is my scripts:
B.T.W: I can run the following scripts in pycharm, but cant run it when package to a single exe using pyinstaller. could anyone can help me

def muscle_main():
muscle_input = dpg.get_value("DPrime_input_fasta")
muscle_output = dpg.get_value("DPrime_msa_output")
os.system("{}/muscle5.1.win64.exe -align {} -output {}".format(os.path.abspath(os.path.join(os.path.dirname(file),"Scripts")),
muscle_input, muscle_output))