diff --git a/.release-please-manifest.json b/.release-please-manifest.json
index 62c031f3f8..37e12350e3 100644
--- a/.release-please-manifest.json
+++ b/.release-please-manifest.json
@@ -1,3 +1,3 @@
 {
-    ".": "3.54.0"
+    ".": "3.55.0"
 }
diff --git a/CHANGELOG.md b/CHANGELOG.md
index ee56542822..d7f8ac42c6 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -4,6 +4,40 @@
 
 [1]: https://pypi.org/project/google-cloud-spanner/#history
 
+## [3.55.0](https://github.com/googleapis/python-spanner/compare/v3.54.0...v3.55.0) (2025-05-28)
+
+
+### Features
+
+* Add a `last` field in the `PartialResultSet` ([d532d57](https://github.com/googleapis/python-spanner/commit/d532d57fd5908ecd7bc9dfff73695715cc4b1ebe))
+* Add support for multiplexed sessions ([#1381](https://github.com/googleapis/python-spanner/issues/1381)) ([97d7268](https://github.com/googleapis/python-spanner/commit/97d7268ac12a57d9d116ee3d9475580e1e7e07ae))
+* Add throughput_mode to UpdateDatabaseDdlRequest to be used by Spanner Migration Tool. See https://github.com/GoogleCloudPlatform/spanner-migration-tool ([d532d57](https://github.com/googleapis/python-spanner/commit/d532d57fd5908ecd7bc9dfff73695715cc4b1ebe))
+* Support fine-grained permissions	database roles in connect ([#1338](https://github.com/googleapis/python-spanner/issues/1338)) ([064d9dc](https://github.com/googleapis/python-spanner/commit/064d9dc3441a617cbc80af6e16493bc42c89b3c9))
+
+
+### Bug Fixes
+
+* E2E tracing metadata append issue ([#1357](https://github.com/googleapis/python-spanner/issues/1357)) ([3943885](https://github.com/googleapis/python-spanner/commit/394388595a312f60b423dfbfd7aaf2724cc4454f))
+* Pass through kwargs in dbapi connect ([#1368](https://github.com/googleapis/python-spanner/issues/1368)) ([aae8d61](https://github.com/googleapis/python-spanner/commit/aae8d6161580c88354d813fe75a297c318f1c2c7))
+* Remove setup.cfg configuration for creating universal wheels ([#1324](https://github.com/googleapis/python-spanner/issues/1324)) ([e064474](https://github.com/googleapis/python-spanner/commit/e0644744d7f3fcea42b461996fc0ee22d4218599))
+
+
+### Documentation
+
+* A comment for field `chunked_value` in message `.google.spanner.v1.PartialResultSet` is changed ([d532d57](https://github.com/googleapis/python-spanner/commit/d532d57fd5908ecd7bc9dfff73695715cc4b1ebe))
+* A comment for field `precommit_token` in message `.google.spanner.v1.PartialResultSet` is changed ([d532d57](https://github.com/googleapis/python-spanner/commit/d532d57fd5908ecd7bc9dfff73695715cc4b1ebe))
+* A comment for field `precommit_token` in message `.google.spanner.v1.ResultSet` is changed ([d532d57](https://github.com/googleapis/python-spanner/commit/d532d57fd5908ecd7bc9dfff73695715cc4b1ebe))
+* A comment for field `query_plan` in message `.google.spanner.v1.ResultSetStats` is changed ([d532d57](https://github.com/googleapis/python-spanner/commit/d532d57fd5908ecd7bc9dfff73695715cc4b1ebe))
+* A comment for field `row_count_lower_bound` in message `.google.spanner.v1.ResultSetStats` is changed ([d532d57](https://github.com/googleapis/python-spanner/commit/d532d57fd5908ecd7bc9dfff73695715cc4b1ebe))
+* A comment for field `row_type` in message `.google.spanner.v1.ResultSetMetadata` is changed ([d532d57](https://github.com/googleapis/python-spanner/commit/d532d57fd5908ecd7bc9dfff73695715cc4b1ebe))
+* A comment for field `rows` in message `.google.spanner.v1.ResultSet` is changed ([d532d57](https://github.com/googleapis/python-spanner/commit/d532d57fd5908ecd7bc9dfff73695715cc4b1ebe))
+* A comment for field `stats` in message `.google.spanner.v1.PartialResultSet` is changed ([d532d57](https://github.com/googleapis/python-spanner/commit/d532d57fd5908ecd7bc9dfff73695715cc4b1ebe))
+* A comment for field `stats` in message `.google.spanner.v1.ResultSet` is changed ([d532d57](https://github.com/googleapis/python-spanner/commit/d532d57fd5908ecd7bc9dfff73695715cc4b1ebe))
+* A comment for field `values` in message `.google.spanner.v1.PartialResultSet` is changed ([d532d57](https://github.com/googleapis/python-spanner/commit/d532d57fd5908ecd7bc9dfff73695715cc4b1ebe))
+* A comment for message `ResultSetMetadata` is changed ([d532d57](https://github.com/googleapis/python-spanner/commit/d532d57fd5908ecd7bc9dfff73695715cc4b1ebe))
+* A comment for message `ResultSetStats` is changed ([d532d57](https://github.com/googleapis/python-spanner/commit/d532d57fd5908ecd7bc9dfff73695715cc4b1ebe))
+* Fix markdown formatting in transactions page ([#1377](https://github.com/googleapis/python-spanner/issues/1377)) ([de322f8](https://github.com/googleapis/python-spanner/commit/de322f89642a3c13b6b1d4b9b1a2cdf4c8f550fb))
+
 ## [3.54.0](https://github.com/googleapis/python-spanner/compare/v3.53.0...v3.54.0) (2025-04-28)
 
 
diff --git a/google/cloud/spanner_admin_database_v1/gapic_version.py b/google/cloud/spanner_admin_database_v1/gapic_version.py
index 9f7e08d550..b7c2622867 100644
--- a/google/cloud/spanner_admin_database_v1/gapic_version.py
+++ b/google/cloud/spanner_admin_database_v1/gapic_version.py
@@ -13,4 +13,4 @@
 # See the License for the specific language governing permissions and
 # limitations under the License.
 #
-__version__ = "3.54.0"  # {x-release-please-version}
+__version__ = "3.55.0"  # {x-release-please-version}
diff --git a/google/cloud/spanner_admin_instance_v1/gapic_version.py b/google/cloud/spanner_admin_instance_v1/gapic_version.py
index 9f7e08d550..b7c2622867 100644
--- a/google/cloud/spanner_admin_instance_v1/gapic_version.py
+++ b/google/cloud/spanner_admin_instance_v1/gapic_version.py
@@ -13,4 +13,4 @@
 # See the License for the specific language governing permissions and
 # limitations under the License.
 #
-__version__ = "3.54.0"  # {x-release-please-version}
+__version__ = "3.55.0"  # {x-release-please-version}
diff --git a/google/cloud/spanner_v1/gapic_version.py b/google/cloud/spanner_v1/gapic_version.py
index 9f7e08d550..b7c2622867 100644
--- a/google/cloud/spanner_v1/gapic_version.py
+++ b/google/cloud/spanner_v1/gapic_version.py
@@ -13,4 +13,4 @@
 # See the License for the specific language governing permissions and
 # limitations under the License.
 #
-__version__ = "3.54.0"  # {x-release-please-version}
+__version__ = "3.55.0"  # {x-release-please-version}
diff --git a/samples/generated_samples/snippet_metadata_google.spanner.admin.database.v1.json b/samples/generated_samples/snippet_metadata_google.spanner.admin.database.v1.json
index 5d2b5b379a..609e70a8c2 100644
--- a/samples/generated_samples/snippet_metadata_google.spanner.admin.database.v1.json
+++ b/samples/generated_samples/snippet_metadata_google.spanner.admin.database.v1.json
@@ -8,7 +8,7 @@
     ],
     "language": "PYTHON",
     "name": "google-cloud-spanner-admin-database",
-    "version": "0.1.0"
+    "version": "3.55.0"
   },
   "snippets": [
     {
diff --git a/samples/generated_samples/snippet_metadata_google.spanner.admin.instance.v1.json b/samples/generated_samples/snippet_metadata_google.spanner.admin.instance.v1.json
index 06d6291f45..c78d74fd41 100644
--- a/samples/generated_samples/snippet_metadata_google.spanner.admin.instance.v1.json
+++ b/samples/generated_samples/snippet_metadata_google.spanner.admin.instance.v1.json
@@ -8,7 +8,7 @@
     ],
     "language": "PYTHON",
     "name": "google-cloud-spanner-admin-instance",
-    "version": "0.1.0"
+    "version": "3.55.0"
   },
   "snippets": [
     {
diff --git a/samples/generated_samples/snippet_metadata_google.spanner.v1.json b/samples/generated_samples/snippet_metadata_google.spanner.v1.json
index 727606e51f..22a0a46fb4 100644
--- a/samples/generated_samples/snippet_metadata_google.spanner.v1.json
+++ b/samples/generated_samples/snippet_metadata_google.spanner.v1.json
@@ -8,7 +8,7 @@
     ],
     "language": "PYTHON",
     "name": "google-cloud-spanner",
-    "version": "0.1.0"
+    "version": "3.55.0"
   },
   "snippets": [
     {