diff --git a/src/tools/crossgen2/Common/TypeSystem/Common/ExplicitLayoutValidator.cs b/src/tools/crossgen2/Common/TypeSystem/Common/ExplicitLayoutValidator.cs
new file mode 100644
index 000000000000..b1c4ae54a09a
--- /dev/null
+++ b/src/tools/crossgen2/Common/TypeSystem/Common/ExplicitLayoutValidator.cs
@@ -0,0 +1,155 @@
+// Licensed to the .NET Foundation under one or more agreements.
+// The .NET Foundation licenses this file to you under the MIT license.
+// See the LICENSE file in the project root for more information.
+
+using System;
+using System.Diagnostics;
+
+namespace Internal.TypeSystem
+{
+    public struct ExplicitLayoutValidator
+    {
+        private enum FieldLayoutTag : byte
+        {
+            Empty,
+            NonORef,
+            ORef,
+        }
+
+        private readonly int _pointerSize;
+
+        private readonly FieldLayoutTag[] _fieldLayout;
+
+        private readonly MetadataType _typeBeingValidated;
+
+        private ExplicitLayoutValidator(MetadataType type, int typeSizeInBytes)
+        {
+            _typeBeingValidated = type;
+            _pointerSize = type.Context.Target.PointerSize;
+            _fieldLayout = new FieldLayoutTag[typeSizeInBytes];
+        }
+
+        public static void Validate(MetadataType type, ComputedInstanceFieldLayout layout)
+        {
+            ExplicitLayoutValidator validator = new ExplicitLayoutValidator(type, layout.ByteCountUnaligned.AsInt);
+            foreach (FieldAndOffset fieldAndOffset in layout.Offsets)
+            {
+                validator.AddToFieldLayout(fieldAndOffset.Offset.AsInt, fieldAndOffset.Field.FieldType);
+            }
+        }
+
+        private void AddToFieldLayout(int offset, TypeDesc fieldType)
+        {
+            if (fieldType.IsGCPointer)
+            {
+                if (offset % _pointerSize != 0)
+                {
+                    // Misaligned ORef
+                    ThrowFieldLayoutError(offset);
+                }
+                SetFieldLayout(offset, _pointerSize, FieldLayoutTag.ORef);
+            }
+            else if (fieldType.IsPointer || fieldType.IsFunctionPointer)
+            {
+                SetFieldLayout(offset, _pointerSize, FieldLayoutTag.NonORef);
+            }
+            else if (fieldType.IsValueType)
+            {
+                if (fieldType.IsByRefLike && offset % _pointerSize != 0)
+                {
+                    // Misaligned ByRefLike
+                    ThrowFieldLayoutError(offset);
+                }
+
+                MetadataType mdType = (MetadataType)fieldType;
+                int fieldSize = mdType.InstanceByteCountUnaligned.AsInt;
+                if (!mdType.ContainsGCPointers)
+                {
+                    // Plain value type, mark the entire range as NonORef
+                    SetFieldLayout(offset, fieldSize, FieldLayoutTag.NonORef);
+                }
+                else
+                {
+                    if (offset % _pointerSize != 0)
+                    {
+                        // Misaligned struct with GC pointers
+                        ThrowFieldLayoutError(offset);
+                    }
+
+                    bool[] fieldORefMap = new bool[fieldSize];
+                    MarkORefLocations(mdType, fieldORefMap, offset: 0);
+                    for (int index = 0; index < fieldSize; index++)
+                    {
+                        SetFieldLayout(offset + index, fieldORefMap[index] ? FieldLayoutTag.ORef : FieldLayoutTag.NonORef);
+                    }
+                }
+            }
+            else if (fieldType.IsByRef)
+            {
+                if (offset % _pointerSize != 0)
+                {
+                    // Misaligned pointer field
+                    ThrowFieldLayoutError(offset);
+                }
+                SetFieldLayout(offset, _pointerSize, FieldLayoutTag.NonORef);
+            }
+            else
+            {
+                Debug.Assert(false, fieldType.ToString());
+            }
+        }
+
+        private void MarkORefLocations(MetadataType type, bool[] orefMap, int offset)
+        {
+            // Recurse into struct fields
+            foreach (FieldDesc field in type.GetFields())
+            {
+                if (!field.IsStatic)
+                {
+                    int fieldOffset = offset + field.Offset.AsInt;
+                    if (field.FieldType.IsGCPointer)
+                    {
+                        for (int index = 0; index < _pointerSize; index++)
+                        {
+                            orefMap[fieldOffset + index] = true;
+                        }
+                    }
+                    else if (field.FieldType.IsValueType)
+                    {
+                        MetadataType mdFieldType = (MetadataType)field.FieldType;
+                        if (mdFieldType.ContainsGCPointers)
+                        {
+                            MarkORefLocations(mdFieldType, orefMap, fieldOffset);
+                        }
+                    }
+                }
+            }
+        }
+
+        private void SetFieldLayout(int offset, int count, FieldLayoutTag tag)
+        {
+            for (int index = 0; index < count; index++)
+            {
+                SetFieldLayout(offset + index, tag);
+            }
+        }
+
+        private void SetFieldLayout(int offset, FieldLayoutTag tag)
+        {
+            FieldLayoutTag existingTag = _fieldLayout[offset];
+            if (existingTag != tag)
+            {
+                if (existingTag != FieldLayoutTag.Empty)
+                {
+                    ThrowFieldLayoutError(offset);
+                }
+                _fieldLayout[offset] = tag;
+            }
+        }
+
+        private void ThrowFieldLayoutError(int offset)
+        {
+            ThrowHelper.ThrowTypeLoadException(ExceptionStringID.ClassLoadExplicitLayout, _typeBeingValidated, offset.ToStringInvariant());
+        }
+    }
+}
diff --git a/src/tools/crossgen2/Common/TypeSystem/Common/MetadataFieldLayoutAlgorithm.cs b/src/tools/crossgen2/Common/TypeSystem/Common/MetadataFieldLayoutAlgorithm.cs
index 79cb2078af80..b998f6dce936 100644
--- a/src/tools/crossgen2/Common/TypeSystem/Common/MetadataFieldLayoutAlgorithm.cs
+++ b/src/tools/crossgen2/Common/TypeSystem/Common/MetadataFieldLayoutAlgorithm.cs
@@ -367,6 +367,8 @@ protected static ComputedInstanceFieldLayout ComputeExplicitFieldLayout(Metadata
             computedLayout.ByteCountAlignment = instanceByteSizeAndAlignment.Alignment;
             computedLayout.Offsets = offsets;
 
+            ExplicitLayoutValidator.Validate(type, computedLayout);
+
             return computedLayout;
         }
 
diff --git a/src/tools/crossgen2/ILCompiler.TypeSystem.ReadyToRun/ILCompiler.TypeSystem.ReadyToRun.csproj b/src/tools/crossgen2/ILCompiler.TypeSystem.ReadyToRun/ILCompiler.TypeSystem.ReadyToRun.csproj
index 2948bc0a2972..0fe25e26ec52 100644
--- a/src/tools/crossgen2/ILCompiler.TypeSystem.ReadyToRun/ILCompiler.TypeSystem.ReadyToRun.csproj
+++ b/src/tools/crossgen2/ILCompiler.TypeSystem.ReadyToRun/ILCompiler.TypeSystem.ReadyToRun.csproj
@@ -114,6 +114,9 @@
     <Compile Include="..\Common\TypeSystem\Common\CastingHelper.cs">
       <Link>TypeSystem\Common\CastingHelper.cs</Link>
     </Compile>
+    <Compile Include="..\Common\TypeSystem\Common\ExplicitLayoutValidator.cs">
+      <Link>TypeSystem\Common\ExplicitLayoutValidator.cs</Link>
+    </Compile>
     <Compile Include="..\Common\TypeSystem\Common\FunctionPointerType.cs">
       <Link>TypeSystem\Common\FunctionPointerType.cs</Link>
     </Compile>