diff --git a/packages/vitest-pool-workers/src/pool/index.ts b/packages/vitest-pool-workers/src/pool/index.ts
index 373d710f93a7..7f36174f3ef2 100644
--- a/packages/vitest-pool-workers/src/pool/index.ts
+++ b/packages/vitest-pool-workers/src/pool/index.ts
@@ -809,6 +809,8 @@ async function getProjectMiniflare(
 		await forEachMiniflare(project.mf, (mf) => mf.setOptions(mfOptions));
 	} else {
 		log.debug(`Reusing runtime for ${project.relativePath}...`);
+		// Set same options to reset DB connections
+		await forEachMiniflare(project.mf, (mf) => mf.setOptions(mfOptions));
 	}
 
 	return project.mf;